Alkyl-, Alkenyl- and alkynylnitroguanidines as cytokinin plant growth regulants

ABSTRACT

There are provided novel branched alkenylnitroguanidine compounds. A method for inducing cytokinin plant growth regulant activity in crop plants by applying to the crop plants certain alkyl-, alkenyl- and alkynyl-nitroguanidine compounds is provided.

This is a continuation of application, Ser. No. 023,348, filed Mar. 9, 1987, now U.S. Pat. No. 4,822,408, issued Apr. 18, 1989, which is a division of Ser. No. 06/759,705 now U.S. Pat. No. 4,677,226, which issued on June 30,1987.

BACKGROUND OF THE INVENTION

This invention relates to certain novel alkenylnitroguanidine compounds. It also relates to a method for inducing cytokinin plant growth regulant responses in crop plants by applying to said crop plants an effective amount of an alkyl-, alkenyl- or alkynylnitroguanidine compound.

Although certain phenyl and benzylnitroguanidines are disclosed by L. M. Speltz et al. in an American Cyanamid Patent Application, Publication Number DE 3345-281 -A1; 84-159658/26, Dec. 20, 1982, said disclosure describes only phenyl and benzylnitroguanidines and phenyl and benzylcyanoguanidines as yield enhancing agents for crops, antilodging agents for grains such as rice and a novel class of cytokinins. The Speltz et al. application does not suggest that other nitroguanidines or cyanoguanidines would exhibit any or all of the above biological activities. Moreover, it fails to disclose or suggest the novel alkenylnitroguanidines of the present invention or to indicate that such compounds would be useful in the clonal propagation of plants.

SUMMARY OF THE INVENTION

The novel alkenylnitroguanidine compounds of this invention may be represented by ##STR1## where R is a branched C₂ -C₆ alkenyl substituent optionally substituted with from one to three, and preferably one or two OH, halogen or C₁ -C₃ alkoxy groups, and the salts, geometric isomers, optical isomers and tautomers thereof.

The present invention also relates to a method for inducing cytokinin responses in plants by applying to the foliage of said plants or to soil containing seeds or other propagating organs thereof, an effective amount of an alkyl-, alkenyl- or alkynylnitroguanidine compound. The compounds suitable for this invention are represented by ##STR2## wherein R¹ is C₁ -C₆ alkyl, optionally substituted with from one to three, and preferably one or two, OH, halogen or C₁ -C₃ alkoxy substituents; C₂ -C₆ alkenyl optionally substituted with from one to three, and preferably one or two OH, halogen or C₁ -C₃ alkoxy substituents or C₂ -C₆ alkynyl optionally substituted with one to three, and preferably one or two, OH, halogen or C₁ -C₃ alkoxy substituents; and the salts geometric isomers, optical isomers and tautomers thereof.

Surprisingly, it has been found that the alkyl-, alkenyl- and alkynylnitroguanidines of this invention provide an improved method for assisting in the in vitro clonal propagation of plants. The alkyl-, alkenyl-and alkynylnitroguanidines of the invention also provide a means for asexually reproducing genetically similar plants and thus a means for the rapid multiplication of new cultivars, possessing improved or differing characteristics.

PREFERRED EMBODIMENT OF THE INVENTION

As cytokinins, the Formula I and II, alkylnitroguanidines, alkenylnitroguanidines and alkynylnitroguanidines produce enhanced growth effects on translocation, with enhanced chlorophyll biosynthesis in some tissues or decreased chlorophyll degradation (senescence) in others.

A preferred group of Formula II compounds for use as cytokinins have the above formula wherein R¹ is C₃ -C₆ alkyl, optionally substituted with one or two OH, halogen or C₁ -C₃ alkoxy; C₄ -C₆ alkenyl, optionally substituted with one or two OH, halogen or C₁ -C₃ alkoxy or C₄ -C₆ alkynyl, optionally substituted with one or two OH, halogen or C₁ -C₃ alkoxy groups.

Salts of the alkyl-, alkenyl- or alkynylnitroguanidine for inducing cytokinin responses in plants when applied in accordance with the method of the present invention, include the inorganic alkali metal, alkaline earth metal, Co, Cu, Zn, and Ag salts, together with the organic amine salts represented by structure, N⁺ R_(a) R_(b) R_(c) R_(d), wherein R_(a), R_(b), R_(c), and R_(d) are each selected from hydrogen and alkyl C₁ -C₃₀ straight or branched chain and optionally substituted with one or two -OH, C₃ -C₆ alkenyl or C₃ -C₆ alkynyl groups. Preferred salts of these compounds include the sodium, calcium, magnesium, potassium, ammonium, methylamine, trimethylamine, dodecylamine, tributylamine, diisopropylamine, triethylamine, tetrabutylamine, and tallow-amine salts.

These salts are readily prepared by dissolving or dispersing the appropriate compound as depicted by Formula II above in an aqueous solution or suspension of, for example, an alkali metal hydroxide, alkaline earth metal hydroxide or organic ammonium hydroxide.

When applying the nitroguanidines of the present invention to foliage of growing crops or the soil in which they have been planted, the formulated compounds are applied to provide about 0.05 kg/ha to about 8.0 kg/ha and preferably about 0.25 kg/ha to 4.0 kg/ha of the alkyl-, alkenyl- or alkynylnitroguanidine.

The compounds of the present invention are effective substitutes for the extensively investigated cytokinin, N⁶ -benzyladenine, in promoting growth of cytokinin dependent soybean callus in tissue culture. Additionally, the cucumber cotyledon bioassay has also been used to demonstrate the cytokinin like activity obtained with the compounds of the invention. As with many hormonetype bioassays, the response is log linear with respect to concentration of compound required to elicit a response.

It is contemplated that the compounds of the present invention will be useful for the treatment of fresh, green fruits and vegetables, ornamental foliage, and fresh, green, newly harvested livestock feed such as alfalfa, sorghum and corn silage, to increase their resistance to deterioration and preserve the color thereof. For use in this treatment the alkyl-, alkenyl or alkynylnitroguanidines of this invention can be formulated as liquid or solid compositions which may be dispersed in a liquid or solid diluent for application to the foliage of the plants, or to the soil in which they are grown. The substituted guanidines of the invention may be formulated as flowable concentrates, emulsifiable concentrates, wettable powders, dusts or dust concentrates. Aqueous sprays containing from about 0.01 to 1000 ppm of alkyl-, alkenyl- or alkynylnitroguanidine are effective for this invention.

A typical emulsifiable concentrate can be prepared by dissolving, on a weight basis, about 8% of the alkyl-, alkenyl- or alkynylnitroguanidine in about 40% of N-methylpyrrolidone, about 35% of a mixture of substituted benzenes, and about 10% of a spreader activator, containing alkylarylpolyoxyethylene glycol, free fatty acid and propanol, with about 7% by weight of a nonionic surfactant, such as octylphenoxy polyethoxy ethanol or nonylphenoxy polyethoxy ethanol. This concentrate may be dispersed in water for application as a dilute liquid spray.

Emulsifiable concentrates can also be prepared by dissolving, on a weight basis, about 10% of the active guanidine in about 58% of N-methylpyrrolidone, about 24% octyl alcohol, and about 8% polyoxyethylated castor oil.

Flowable liquid concentrates can be prepared by grinding together about 35%, by weight, of the nitroguanidine, about 0.40% colloidal magnesium aluminum silicate, about 1.50% sodium salts of polymerized alkyl naphthalene sulfonic acids, about 8.0% propylene glycol, about 0.1% ethoxylated octylphenol, about 0.1% nonylphenoxy polyethoxy ethanol, about 0.07% citric acid, about 0.06% xanthan gum, about 0.10% paraformaldehyde and about 54.77% water.

Flowable liquid concentrates can also be prepared by milling together about 43%, by weight, of the nitroguanidine with about 0.4%, by weight, of colloidal magnesium aluminum silicate, about 1.5%, by weight, of naphthalene formaldehyde condensate, about 8%, by weight, of polyethylene glycol, about 0.1%, by weight, of nonylphenol ethylene oxide condensate (9-11 moles ethylene oxide), about 0.1%, by weight, of a dispersing agent (e.g., sodium lignosulfonate), about 0.07%, by weight, citric acid, about 49%, by weight, of water, and about 0.06%, by weight, of xanthan gum. This concentrate is dispersed in water for application as a liquid spray.

A typical wettable powder can be prepared by grinding together, on a weight basis, about 20 to 45% of a finely-divided carrier (e.g., kaolin, bentonite, diatomaceous earth and attapulgite, about 45 to 80% of the alkyl or alkenylnitroguanidine, about 2 to 5% of a dispersing agent (e.g., sodium lignosulfonate), and about 2 to 5% of a nonionic surfactant (e.g., octylphenoxy polyethoxy ethanol or nonylphenoxy polyethoxy ethanol). This formulation is generally dispersed in water for application as a liquid spray.

Advantageously, the alkyl-, alkenyl- and alkynylnitroguanidines encompassed by Formulas I and II are useful in the methods of the present invention and can be prepared by reaction of an appropriately substituted aniline with approximately an equimolar amount of an N-alkyl-N-nitroso-N'-nitroguanidine in the presence of an aqueous alcoholic solution. The mixture is heated to about 40° C., treated with a strong base such as sodium hydroxide, and the alcohol removed from the mixture by evaporation. The remaining liquid is then filtered, and the filtrate acidified with a strong mineral acid (i.e., hydrochloric acid) to yield the desired nitroguanidine. This reaction is graphically illustrated as follows: ##STR3## wherein R¹ is alkyl C₁ -C₆, alkenyl C₂ -C₆ or alkynl C₂ -C₆ and each of said alkyl, alkenyl or alkynyl function is straight or branched and each is optionally substituted with halogen, OH or alkoxy C₁ -C₃ groups.

Nutrient media suitable for use in the in vitro propagation of plants may comprise a mixture of various mineral salts, vitamins, amino acids, a carbohydrate source, and biological agents and hormones such as gibberellins and auxins, depending upon the cultivar or plant species which is being propagated. For example, it is a well known phenomenon that both embryogenesis and shoot/root induction depend on both the levels and ratios of auxins and cytokinins in the inductive medium. The cultivar or plant species is of course an important factor to consider in that certain species, cultivars, individuals or plant parts are capable of producing some of the necessary factors even while in tissue culture. A semisolid supporting medium such as agar or an aqueous medium may be used. When auxins are used in the inductive medium they usually must be present at relatively low levels to allow expression of the embryo and shoot inducing phenomena.

The preferred concentration of Formula I and Formula II compounds for use in the in vitro propagation of plants are on the order of 10⁻⁵ to 10⁻⁴ moles per liter of compound. These concentrations are dependent on factors such as the species or cultivar employed, the origin of the tissue to be propagated, and additional components in the medium. With certain of the compounds, primarily those which have activity at low concentrations, the use of the high concentrations may be deleterious to subsequent shoot or embryo development and should be avoided. Such a phenomenon is often seen when hormones or hormone-substitutes are employed by biological systems.

The following examples are presented for the purpose of illustrating the present invention and facilitating a better understanding thereof.

EXAMPLE 1 l Preparation of 1-(3-methyl-2-butenyl)-3-nitroguanidine

3-methyl-2-butenylamine (3.0 g) is added dropwise at room temperature to a slurry of 1-methyl-3-nitro-1 -nitrosoguanidine (4.3 g) in 50 ml of EtOH/H₂ O (1/1). After stirring the mixture at room temperature for 18 hours, the precipitate which forms is removed by filtration. The solid is then slurred with dilute hydrochloric acid, collected by filtration and washed with ethanol/water (30/70). Recrystallization from ethanol gives a white solid with mp 134°-136° C.

Following the above procedure but substituting the appropriate amine for 3-methyl-2-butenyl amine yields the compounds listed in Table I below. The reaction may be illustrated as shown in Table I.

                  TABLE 1                                                          ______________________________________                                          ##STR4##                                                                      R                     MP °C.                                            ______________________________________                                          -n-C.sub.5 H.sub.11  101-102                                                  CH.sub.2CHCH.sub.2    110-112                                                  C.sub.2 H.sub.5 OCH.sub.2 CH.sub.2                                                                   86.5-88.5                                                (CH.sub.3).sub.2 CHCH.sub.2 CH.sub.2                                                                 145-148                                                   ##STR5##             124-127                                                   -n-C.sub.4 H.sub.9   86.0-87.5                                                HCCC(CH.sub.3).sub.2  112-113                                                  CH.sub.3 OCH.sub.2 CH(CH.sub.3)                                                                      101-104                                                  (CH.sub.3).sub.2 CH   158-160                                                  C.sub.2 H.sub.5 C(CH.sub.3).sub.2                                                                    137.5-139                                                CH.sub.2CHC(CH.sub.3).sub.2                                                                          122.5-123.5                                               ##STR6##             116-121                                                  HOCH.sub.2 CH.sub.2 CH.sub. 2 CH.sub.2                                                               113-116                                                   ##STR7##             134-136                                                   ##STR8##              92-100                                                   ##STR9##             129-131                                                   ##STR10##              106-107.5                                              CH.sub.3              161.5-163                                                 -n-C.sub.3 H.sub.7     98-98.5                                                (CH.sub.3).sub.3 C    200-203                                                   ##STR11##            yellow oil                                                ##STR12##            121-125                                                   ##STR13##            100-104                                                   ##STR14##            138-140                                                  ______________________________________                                    

EXAMPLE 2 Bioassay for relative cytokinin activity using the cucumber cotyledon test

The compounds of this invention possess plant hormone activity of the class referred to as cytokinins. In view of this, the relative activity of these compounds is determined by a modification of the cucumber cotyledon greening bioassay (Fletcher et al. Plant Physiology (1982) 69:695-77).

Cucumber seeds (Cucumis sativa var. National Pickling) are germinated in vermiculite in the dark at 300° C. for 6 days. The cotyledons are subsequently excised under a dim green safelight and placed abaxial side up in 10×100 cm petri dishes at 8 pairs of cotyledons per dish. The dishes also contain three layers of Whatman No.1 filter paper and 10 mls of test solution. The dishes with cotyledons are returned to the dark at 30° C. for about 18 hours. Thereafter the dishes are placed under fluorescent light at about 12μw per square cm for 3 hours at 25° C. The cotyledons are then blotted and placed in 5 ml of dimethylformamide overnight and at 40° C. to extract the chlorophyll. The leachate is then analyzed for absorbance at 663 nanometers by spectrophotometry (Moran and Porath Plant Physical (1980) 65:478-9) to determine relative chlorophyll biosynthesis. The cotyledons are then measured for area by using a sensitive leaf area meter. These two parameters, chlorophyll synthesis and enhanced expansion of the cotyledons are used as a measure of relative cytokinin activity. The test solutions typically contain between 0.06 and 64 ppm of test compound, up to 1.3% acetone, and 40 mM KCl. The amount of compound is varied in order to determine the dose at which there is achieved a response which is 50% of the maximum possible enhancement, as judged by a standard. This is termed the 50% effective dose or ED50 (Table). The compound is judged as inactive if the amount required is more than 200 ppm. Date obtained are reported in Table below.

                  TABLE II                                                         ______________________________________                                          ##STR15##                                                                     R                    ED.sub.50, ppm                                            ______________________________________                                          ##STR16##           31                                                        CH.sub.3             113                                                       (CH.sub.3).sub.3 C   31                                                        (CH.sub.3).sub.2 CCHCH.sub.2                                                                        3.8                                                        -n-C.sub.5 H.sub.11 31                                                        CH.sub.2CHCH.sub.2   22                                                        C.sub.2 H.sub.5 OCH.sub.2 CH.sub.2                                                                  113                                                       (CH.sub.3).sub.2 CHCH.sub.2 CH.sub.2                                                                64                                                         ##STR17##           1.0                                                        -n-C.sub.4 H.sub.9  31                                                        CH.sub.3 OCH.sub.2 CH(CH.sub.3)                                                                     113                                                       (CH.sub.3).sub.2 CH  45                                                        C.sub.2 H.sub.5 C(CH.sub.3).sub.2                                                                   22                                                        CH.sub.2CHC(CH.sub.3).sub.2                                                                         157                                                        ##STR18##           14                                                        HOCH.sub.2 CH.sub.2 CH.sub.2 CH.sub.2                                                               113                                                       (Cl).sub.2 CCHCH.sub.2 CH.sub.2                                                                     11                                                         ##STR19##           2.5                                                       ______________________________________                                    

EXAMPLE 3 Definitive Cytokinin Activity in the Soybean Callus Culture Bioassay

Soybean callus cultures (from Glycine max CV. Acme) are subdivided into smaller callus pieces each weighing about 80 mg each. These pieces are placed on 50 ml of agar containing nutrient medium from which the usual growth supporting cytokinin hormone has been deleted. Without cytokinin hormone, the callus tissue will not divide and grow. Any compound which can provide for growth of the callus under these circumstances is by definition a cytokinin. The test is thus definitive and diagnostic. Different flasks of the nutrient medium are therefore supplemented with either the known cytokinin N⁶ -benzyladenine as a standard or with the indicated test compound. After 6 weeks of axenic culture, the callus pieces are weighed, then oven dried and weighed again, such that the amount of callus growth is determined as a function of increased tissue weight. The optimum concentration of the standard, N⁶ benzyladenine, is 10⁻⁶ M with 10⁻⁴ M giving a supraoptimal effect whereby the beneficial effect was negated. The compound 1-(3-methyl-2-butenyl)-3-nitroguanidine is extremely active at 10⁻⁴ M as a cytokinin in this test. Data obtained are reported in Table III below.

                  TABLE III                                                        ______________________________________                                         Cytokinin Activity of Test Compound                                            in Soybean Callus Growth Test                                                            mg per 3 pieces                                                      Treatment*  Callus Fresh Weight                                                                           Callus Dry Weight                                   ______________________________________                                         No supplement                                                                              283 ± 99    22.2 ± 9                                         1-(3-methyl-2-                                                                             5324 ± 1148 410 ± 87                                         butenyl)-3-nitro-                                                              guanidine 10.sup.-4  .sub.-- m                                                 N.sup.6 -benzyladenine                                                                     5826 ± 1110 351 ± 63                                         10.sup.-6  --M                                                                 N.sup.6 benzyladenine,                                                                     434 ± 193   28 ± 5                                           10.sup.-4  --M                                                                 ______________________________________                                          *Initial fresh weight was 240 g; 3 flasks were used per treatment        

EXAMPLE 4 Antagonism of Cytokinin Activity By 7-(Isopentylamine)-3 -methyl-1H-Pyrazolopyrimidine Using the Cucumber Cotyledon Bioassay

The above compound is a known (Hecht el al.) Biochemistry 10:4224 (1971) antagonist of the activity of cytokinins such as the natural cytokinin 6-(-3-methyl2-butenylamino)purine (2iP). For example 0.003 μM 2iP is required for maximum growth of tobacco callus tissue cultures. The antagonist decreases this effect in the range of 0.1 μM (detectable) to 0.73 μM (lethal). (Ref. Skoog et al. Phytochemistry 12:25-37 (1973)).

The antagonist also prevents the response of cucumber cotyledons to the known synthetic cytokinin N⁶ -benzyladenine, indicating that it is also an antagonist of cytokinin in the chlorophyll-synthesis response of the cucumber bioassay. Test solutions are prepared to contain a test compound in both the presence and absence of the antagonist. The cytokinin antagonist is able to fully prevent the enhancement of chlorophyll synthesis in the cucumber cotyledon test (Table). Data obtained are reported in Table IV below.

                  TABLE IV                                                         ______________________________________                                         Antagonism of 1-(3-methyl-2-butenyl)-3-nitroguanidine                          By a Cytokinin Antagonist In the Cucumber Cotyledon Bioassay                                                Chlorophyll                                       Treatment         Relative   Increase,                                         Test Compound                                                                              Antagonist                                                                               Chlorophyll                                                                               % of Maximum                                  ______________________________________                                         H.sub.2 O   None      370        0                                             H.sub.2 O   10 ppm    415        9.3                                           N.sup.6 -benzylamino-                                                                      None      853        100                                           purine, 0.1 ppm                                                                N.sup.6 -benzylamino-                                                                      10 ppm    460        18.7                                          purine, 0.l ppm                                                                B                                                                              H.sub.2 O   None      443        0                                             H.sub.2 O   10 ppm    406        4.9                                           1-(3-methyl-2-                                                                             None      1200       100                                           butenyl)-3-nitro-                                                              guanidine, 20 ppm                                                              1-(3-methyl-2-                                                                             10 ppm    388        -7.3                                          butenyl)-3-nitro-                                                              guanidine, 20 ppm                                                              ______________________________________                                    

EXAMPLE 5 Efficacy of the use of guanidine compounds for assisting in stimulation of callus growth in habituated callus tissue

A specialized callus tissue (Nicotiana tabacum A21-1OH) which was known to be unique in that it had been selected to no longer require cytokinins for callus to grow (this condition is known as "habituated") as long as 2 ppm naphthalene acetic acid was present in the medium, is transferred to flasks of basal media containing mineral salts based on the formulation of Murashige and Skoog, Physiologia Plantarum 15:473-497 (1962), and containing 100 mg per liter of i-inositol, 0.4 mg per liter of thiamine.HCl, and 3% sucrose (Huang, L.C. and T. Murashige 1976, TCA manual 3(1):539-548) as well as 0.9% to 0.95% Difco® agar. The pH of the medium is adjusted to pH 5.8.

For purposes of demonstration the efficacy of use of the guanidine compounds, the basal medium is also supplemented with only 0.02 ppm naphthalene acetic acid (i.e., 1% of the usual level). Test solutions are added from 5000 ppm stock solutions in ethanol and the stimulation of callus growth determined by the increase in fresh weight per treatment (per flask) in grams and by chlorophyll production rated as indicated in the rating system below. (No shoot regeneration can occur in this line due to the length of time (>4 years) it has been maintained in culture.)

Chlorophyll Production Rating System

    ______________________________________                                         Chlorophyll Production Rating System                                           ______________________________________                                         -                   None                                                       +                   Slight                                                     ++                  Moderate                                                   +++                 Very Good                                                  ______________________________________                                    

The results of these experiments are summarized in Table V below. These results demonstrate

1. the efficacy of the use of the alkenylnitroguanidines of the invention for assisting in callus multiplication and chlorophyll production, and

2. that the use of these compounds is unique in comparison to kinetin, a cytokinin which is ineffective in this system due to the resuction of the auxin level.

                  TABLE V                                                          ______________________________________                                         Stimulation of Callus Growth From A                                            Habituated line of Nicotiana tabacum                                                           Callus Growth                                                           Molar        Fresh weight,                                                                              Chlorophyll                                  Treatment                                                                               Concentration                                                                               grams per flask                                                                            production                                   ______________________________________                                         None     --           0.6         -                                            Kinetin  10.sup.-7    0.2         -                                            (standard)                                                                              10.sup.-6    0.3         -                                                     10.sup.-5    0.8         -                                                     10.sup.-4    1.4         -                                            1-(3-methyl-                                                                            10.sup.-4    2.9         ++                                           2-butenyl)-                                                                    3-nitro-                                                                       guanidine                                                                      ______________________________________                                     

What is claimed is:
 1. A method for inhibiting deterioration of fresh, leafy vegetables, ornamental foliage and fresh, green, newly harvested livestock feed, comprising applying thereto a preservative amount of a substituted nitroguanidine compound, wherein the nitroguanidine compound is ##STR20## wherein R¹ is C₁ -C₆ alkyl, optionally substituted with from one to three OH, halogen or C₁ -C₃ alkoxy groups; C₂ -C₆ alkenyl optionally substituted with from one to three OH, halogen or C₁ -C₃ alkoxy groups or C₂ -C₆ alkynl, optionally substituted with from one to three OH, halogen or C₁ -C₃ alkoxy groups; or the salts, geometric isoners, optical isomers or tautomers thereof.
 2. The method according to claim 1, wherein said nitroguanidine compound is applied as a dilute formulation containing from about 0.01 to 1000 ppm of said nitroguanidine compound.
 3. The method according to claim 2, wherein said compound is 1-(3-methyl-2-butenyl)-3-nitroguanidine.
 4. The method according to claim 1, wherein said compound is 1-(3-chloro-2-butenyl)-3 -nitroguanidine. 